Verify your dsRNA

1. dsCheck accepts FASTA format or flat nucleotide sequence as an input. Users can also enter an accession number to retrieve a sequence from GenBank. In the input sequence, characters other than A, T, G, C, U, N, R, Y, M, K, S, W, H, B, V and D are ignored. U is replaced with T, and letters other than A, T, G, C and U are replaced with Ns. Both lower-case and upper-case letters are accepted. Current limit of the input sequence is 3000 bp.
2. Choose your species. Currently, RefSeq mRNA sequences for Drosophila, C. elegans, Arabidopsis and Oryza sativa are available.
3. Click 'verify' to show off-target gene candidates.

   

4. Suspected off-target gene candidates are listed in few seconds. Number of hits with a complete match (19/19 matches), one mismatches (18/19 matches), or two mismatches (17/19) are counted for every off-target gene candidates individually. In this example, significant hits against two splicing variants of pdm2 (maybe your intended target gene), and two other genes nub and vvl (these seem to be off-targets). This result indicates a high risk of cross-suppressing nub and vvl by your dsRNA. Intended target genes (NM_078834.2, pdm2 and , NM_165017.1pdm2) are highlighted.
5. The result can be downloaded as a 'Tab-Text' file.